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Sheffield University
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Porous elastomeric polymer scaffolds for 3D in vitro cell culture.

It is increasingly understood that 2D in vitro cell culture is not representative of the 3D environment that many cells experience in nature, such as mammalian cells growing in tissues. Non-physiological 2D culture affects cell health, behaviour, and function.

These poly(glycerol sebacate) methacrylate (PGS-M) porous scaffolds support 3D in vitro cell culture. This permits more physiologically relevant laboratory investigations. The scaffolds may also be used as high surface area substrates for adherent cells in bioreactor culture.

PGS-M scaffolds are highly porous and biocompatible. PGS-M is elastomeric, with a Young’s modulus of ~1.5 MPa. This makes the PGS-M scaffolds far more compliant than alternatives available on the market which are produced from stiffer materials, such as polystyrene. The PGS-M scaffolds are disk-shaped and available in sizes to fit standard 48 well tissue culture plates.


PGS-M 3D cell culture scaffold

Poly(glycerol sebacate) methacrylate 3D cell culture scaffold

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